Great Britain 3 - 0 - Hydrosulphato 4 hydroxyphenethylamine ( Dopanine 3 - 0 - Sulphate ) , a Metabolite Involved in the Sclerotization of Insect Cuticle
نویسندگان
چکیده
Dopamine 3-0-sulphate (3-0-hydrosulphato-4-hydroxyphenethylamine) was isolated from newly ecdysed cockroaches, Periplaneta americana (L.), and its structure established by chemical and physical techniques and by synthesis. Relatively high concentrations (about 1 pmol/g wet wt.) of dopamine 3-0-sulphate exist in the newly ecdysed insect, and these concentrations decrease sharply as sclerotization of the cuticle proceeds. At least 40% of the radioactivity of "C-labelled dopamine 3-0-sulphate injected into newly ecdysed nymphs was recovered in the sclerotized cuticle 7-12 days after the injection. However, less than 1% of the radioactivity of injected dopamine 3-O-[35S]sulphate was recovered, and this value was not appreciably different from that for the incorporation of Na235SO4. Apparently, little or none of the sulphate moiety of dopamine 3-0-sulphate is incorporated directly into the cuticle as the intact sulphate ester. These observations are discussed in relation to current concepts of cuticular sclerotization in insects.
منابع مشابه
Pupal cuticle proteins of Manduca sexta: characterization and profiles during sclerotization.
Proteins in pupal abdominal cuticle of the tobacco hornworm, Manduca sexta, were characterized during the pre-ecdysial and post-ecdysial periods of sclerotization and endocuticle formation. Protein extractability decreased dramatically as the cuticle became sclerotized through 6 h post-ecdysis, but increased rapidly from 9 to 48 h as endocuticular layers were secreted. Nearly 100 proteins that ...
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